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Introduction

This repository contains the code required to reproduce the results from the manuscript ‘Spatial omics of acute myocardial infarction reveals a novel mode of immune cell infiltration’. The repository is organized as follows:

Data setup

All scripts in this repository assume that this repository is in the same parent folder as the /data directory, containing all the data on Synapse. Data directory structure can be set up using the Synapse command-line interface with the following command, which will download all of the raw data to your local drive.

`synapse get -r syn51449054 `

For more options to download the data within Synapse, you can select Download Options in the Synapse project and check out Programmatic options. This will allow to also download the data directly using R and Python.

You can then clone this repository alongside the data repository from Synapse:

git clone https://github.com/FloWuenne/mi_spatialomics.git

Data processing

Pipelines used to process the imaging data

Imaging data in this study was processed using Nextflow pipelines designed for each specific data type (nf-core/molkart -> Molecular Cartography, MCMICRO -> SeqIF). Links to the original pipeline repositories is provided below. Config files and specifications for running each pipeline to process the data is provided in this repository under : ./pipeline_configs

  • Processing of Molecular Cartography was done using nf-core/molkart.
  • Processing of SeqIF (Lunaphore COMET) data was done using MCMICRO.

For more details on data processing, go to Data processing.

Data availability

All raw images and processed data is available from Synapse: Spatial omics of acute myocardial infarction reveals immune cell infiltration via the endocardial layer.

Preprint (bioRxiv)

Please cite our preprint if you are using any of the data produced in this study:

Spatial omics of acute myocardial infarction reveals a novel mode of immune cell infiltration

doi: https://doi.org/10.1101/2024.05.20.594955